Scientific research : Upregulated GPRC5A disrupting the Hippo pathway promotes the proliferation and migration of pancreatic cancer cells via the cAMP-CREB axis | ES/IODE
Document detail
ID

doi:10.1007/s12672-023-00626-1...

Author
Fang, Weidan Yu, Xin Deng, Jun Yu, Bin Xiong, Jianping Ma, Mei
Langue
en
Editor

Springer

Category

Medicine & Public Health

Year

2023

listing date

2/8/2023

Keywords
pancreatic cancer gprc5a hippo pathway yap1 camp-creb pancreatic cancer gprc5a
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Abstract

Background Pancreatic cancer has a high mortality rate worldwide, and is predicted to be third leading cause of death in the near future.

However, the regulatory mechanisms that inhibit the progression of pancreatic cancer remain elusive.

Currently, exploring the function and mechanisms of GPCRs (G-protein coupled receptors) is an important way to discover promising therapeutic targets for cancer.

Methods GPRC5A expression was measured using real-time quantitative PCR, immunohistochemistry and western blot assays.

Cell proliferation and migration were assessed using CCK-8, clone formation, wound-healing and transwell assays.

A cytosolic/nuclear distribution experiment was used to detect the protein location transfer.

A xenograft model of pancreatic cancer was established to explore the role of GPRC5A in vivo.

Results GPRC5A expression was increased in pancreatic cancer, and disruption of GPRC5A expression inhibited tumor growth in vivo.

Mechanistically, GPRC5A positively regulated the transcription of YAP1 through cAMP-CREB signaling.

Moreover, we show that the proliferation and migration induced by GPRC5A in pancreatic cancer could be rescued by inhibiting YAP1 expression.

Conclusions GPRC5A interacts with the Hippo pathway to promote the progression of pancreatic cancer.

These findings reveal an important crosstalk model and provide potential targets for pancreatic cancer therapy.

Fang, Weidan,Yu, Xin,Deng, Jun,Yu, Bin,Xiong, Jianping,Ma, Mei, 2023, Upregulated GPRC5A disrupting the Hippo pathway promotes the proliferation and migration of pancreatic cancer cells via the cAMP-CREB axis, Springer

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